Proteolytic

Neutral Protease

An endopeptidase that cleaves internal peptide bonds in partially digested proteins. Acting after gastric proteases have initiated protein breakdown, it further reduces polypeptide chains into smaller fragments, complementing overall protein digestion and supporting complete amino acid release.*

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ENZYME CLASSIFICATION

EC NUMBER | CAS NUMBER:
3.4.24.28 | 76774-43-1
ENZYME CLASS:
Hydrolase → Peptidase
ENZYME TYPE:
Metalloprotease
ENZYME SOURCE:
Bacillus amyloliquefaciens
Enzyme Applications:
Dietary Supplements, Animal Nutrition, Food & Beverage, Brewing & Distilling, Industrial Processing, and Cleaning

TECHNICAL SPECIFICATIONS

ACTIVITY MEASUREMENT:
PC (Protease Units on Casein)
PRIMARY SUBSTRATE:
Partially digested dietary proteins
BOND CLEAVED:
Internal peptide bonds
PRIMARY PRODUCTS:
Peptides, oligopeptides
INACTIVATION:
pH ≤ 4 for 30 minutes
FORM:
Powder
SOLUBILITY:
Water-soluble
STORAGE:
Store in a cool, dry environment
SHELF-LIFE:
18 months from MFG date if properly stored.
SAFETY & HANDLING:
Always refer to the included Safety Data Sheet.
Neutral Protease
Neutral Protease

BIOCHEMICAL ROLE IN DIGESTION

Neutral protease continues proteolysis after gastric initiation, acting on partially hydrolyzed polypeptides in the small intestine. With broad but balanced specificity, it trims internal peptide bonds to generate mid-size fragments that are ideal substrates for brush-border and luminal exopeptidases. This mid-phase action is pivotal for sustaining proteolytic throughput, ensuring polypeptides are efficiently handed off to finishing enzymes for rapid conversion to absorbable nitrogenous end-products.*

NUTRITIONAL RELEVANCE IN SUPPLEMENTATION

In supplementation, neutral protease complements early gastric cleavage and supports the intestinal phase when pancreatic output is modest or meal complexity is high. By reducing the persistence of large polypeptides, it promotes more complete amino acid liberation and helps minimize discomfort associated with slow protein breakdown. Its mid-phase positioning makes it especially useful in blends formulated for mixed meals and variable digestive capacity.*