Why pHysioProtease®

By choosing to use proteases that work best in the blood and tissue fluid, despite the additional cost, pHysioProtease® remains the most effective systemic protease blend available for modulating the inflammatory response and supporting immune and cardiovascular functions.* pHysioProtease® consists of highly purified proteolytic enzymes concentrated from non-genetically modified microorganisms, primarily Aspergillus oryzae and Aspergillus niger, with a long history of safe use. There are many types of protease derived from these microbial

physio protease chart

*mHUT or modified HUT is the designation given for the proteolytic activity expected in the blood and tissue fluid.  This unit is based on modifying the temperature, pH, substrate and buffering mechanism of the standard FCC HUT assay to simulate the conditions found in the blood.  Products A, B, and C may have high standard FCC HUT values but this merely indicates they are optimally active in the digestive tract not in the blood and tissue fluids.

sources, each with its own set of parameters for optimal activity such as temperature, pH, substrate specificity, cleavage pattern, etc. The protease most commonly found in both digestive and systemic enzyme supplements is one that works best in the physiological conditions of the digestive tract as it is intended to break down dietary protein into smaller peptides and amino acids. The industry standard for measuring the activity of this protease uses FCC or Food Chemical Codex methodology based on gastrointestinal parameters. However, the physiological conditions of blood and tissue fluid are significantly different than those of the digestive tract so the standard HUT measurement does not reflect the true activity of a protease in these body fluids. Most notably, the standard FCC assay is run at a pH of 4 whereas the pH of the blood is 7.4.[50] Aware of this disparity, an innovative study was conducted by Enzymes, Inc. to measure proteolytic activity of different commercially-available systemic protease supplements adjusting the FCC testing methodology to reflect blood rather than gastrointestinal parameters. The results revealed pHysioProtease® provides up to 52 times more activity than other proteases used for systemic purposes. In addition, pHysioProtease® has repeatedly be found to enhance the health-promoting properties of herbs, vitamins, minerals and phytonutrients by helping to maintain a normal inflammatory response.*


  1. Food Chemical Codex, 12th edition.
Close Menu